The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 25, 2018

Filed:

Jul. 10, 2017
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Adam Ezra Cohen, Cambridge, MA (US);

Joel Kralj, Louisville, CO (US);

Adam D. Douglass, Salt Lake City, UT (US);

Daniel Hochbaum, Cambridge, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/566 (2006.01); G01N 33/543 (2006.01); A61K 49/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/566 (2013.01); A61K 49/0017 (2013.01); A61K 49/0097 (2013.01); G01N 33/54373 (2013.01); G01N 2333/705 (2013.01);
Abstract

The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.


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