The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 25, 2018
Filed:
Jun. 14, 2017
Applicant:
President and Fellows of Harvard College, Cambridge, MA (US);
Inventors:
Alexei Finski, Somerville, MA (US);
Gavin MacBeath, Boston, MA (US);
Assignee:
PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); B01J 19/00 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
B01J 19/0046 (2013.01); G01N 33/5091 (2013.01); G01N 33/6803 (2013.01); G01N 33/6848 (2013.01); B01J 2219/0061 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00626 (2013.01); B01J 2219/00691 (2013.01); B01J 2219/00695 (2013.01); B01J 2219/00702 (2013.01); B01J 2219/00725 (2013.01);
Abstract
The invention provides a method for lysing a single cell embedded in a tissue from the inside of the cell, and collecting the intracellular lysate for use in analytical methods. This method preserves the state of molecules of the cell, and therefore allows for transformation of a single target cell in live tissue into a format that can be evaluated using analytical methods.