The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 18, 2018

Filed:

Sep. 12, 2016
Applicant:

The Governors of the University of Alberta, Edmonton, CA;

Inventors:

Hongquan Zhang, Edmonton, CA;

Xiaochun Le, Edmonton, CA;

Xing-Fang Li, Edmonton, CA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/115 (2010.01); C07K 14/47 (2006.01); C07K 14/49 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); A61K 38/00 (2006.01);
U.S. Cl.
CPC ...
C07K 14/49 (2013.01); C07K 14/47 (2013.01); C12N 9/22 (2013.01); C12N 15/111 (2013.01); C12N 15/115 (2013.01); A61K 38/00 (2013.01); C12N 2310/16 (2013.01); C12N 2320/32 (2013.01);
Abstract

The invention provides a binding-induced DNA nanomachine that can be activated by proteins and nucleic acids. This new type of nanomachine hamesses specific target binding to trigger assembly of separate DNA components that are otherwise unable to spontaneously assemble. Three-dimensional DNA tracks of high density are constructed on gold nanoparticles functionalized with hundreds of single-stranded oligonucleotides and tens of an affinity ligand. A DNA swing arm, free in solution, can be linked to a second affinity ligand. Binding of a target molecule to the two ligands brings the swing arm to AuNP and initiates autonomous, stepwise movement of the swing arm around the AuNP surface. The movement of the swing arm generates hundreds of oligonucleotides in response to a single binding event. The new nanomachines have several unique and advantageous features over DNA nanomachines that rely on DNA self-assembly.


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