The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 18, 2018

Filed:

Jan. 27, 2016
Applicant:

Yale University, New Haven, CT (US);

Inventors:

Peter M. Rabinovich, Madison, CT (US);

Sherman M. Weissman, New Haven, CT (US);

Marina E. Komarovskaya, Milford, CT (US);

Erkut Bahceci, Hamden, CT (US);

Samuel Katz, Woodbridge, CT (US);

Efim Golub, East Haven, CT (US);

Assignee:

Yale University, Hew Haven, CT (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 9/66 (2006.01); A61K 39/395 (2006.01); C12N 15/85 (2006.01); A61K 45/06 (2006.01); A61K 48/00 (2006.01); C12N 5/074 (2010.01); A61K 39/00 (2006.01); C12N 5/0781 (2010.01); C12N 5/0783 (2010.01); A61K 35/15 (2015.01); A61K 35/17 (2015.01);
U.S. Cl.
CPC ...
A61K 39/39558 (2013.01); A61K 35/15 (2013.01); A61K 35/17 (2013.01); A61K 39/0011 (2013.01); A61K 45/06 (2013.01); A61K 48/005 (2013.01); C12N 5/0635 (2013.01); C12N 5/0638 (2013.01); C12N 5/0646 (2013.01); C12N 5/0696 (2013.01); C12N 15/85 (2013.01); A61K 2039/5156 (2013.01); A61K 2039/5158 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/606 (2013.01); C12N 2506/094 (2013.01); C12N 2506/1307 (2013.01); C12N 2510/00 (2013.01); C12N 2830/008 (2013.01);
Abstract

Compositions and methods of making cells using RNA, and cells made using the disclosed compositions and methods are also provided. In exemplary embodiments, RNA is transfected into cells to effect a molecular, biological, physiological, or histological change in the cells. In preferred embodiments, the RNA is prepared in vitro, more preferably using a DNA template according to the provided compositions and methods. Methods for treating or inhibiting a disorder or disease such cancer are also provided. The methods can include, for example, locally or systemically administering to the host an effective amount of one or more RNAs; or an effective amount of population of cells isolated from the subject or a syngeneic or histocompatible subject, contacted ex vivo with one or RNAs, and optionally expanded. The cells can be, for example, immune cells or stem cells.


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