The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 11, 2018
Filed:
Mar. 05, 2015
Adaptive Biotechnologies Corporation, Seattle, WA (US);
Fred Hutchinson Cancer Research Center, Seattle, WA (US);
Harlan Saul Robins, Seattle, WA (US);
Christopher Scott Carlson, Kirkland, WA (US);
Mark J. Rieder, Seattle, WA (US);
Anna M. Sherwood, Seattle, WA (US);
Ryan O. Emerson, Seattle, WA (US);
ADAPTIVE BIOTECHNOLOGIES CORP., Seattle, WA (US);
Abstract
A relative representation of adaptive immune cells in a biological sample is quantified using multiplex PCR and sequencing of adaptive immune cells, control genes, and synthetic template molecules. Disclosed herein are methods for quantifying a number of adaptive immune cells in a biological sample, and methods for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells. Methods are provided for amplifying by multiplex PCR and sequencing a first set of synthetic templates each comprising one TCR or IgV segment and one TCR of Ig J or C segment and a unique bar code.