The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 11, 2018
Filed:
Apr. 09, 2009
David H. Gelfand, Oakland, CA (US);
Ivo Glynne Gut, Paris, FR;
Keith A. Bauer, San Rafael, CA (US);
Florence Mauger, Evry, FR;
David H. Gelfand, Oakland, CA (US);
Ivo Glynne Gut, Paris, FR;
Keith A. Bauer, San Rafael, CA (US);
Florence Mauger, Evry, FR;
Roche Molecular Systems, Inc., Pleasanton, CA (US);
CEA/Institut de Genomique—Centre National de Genotypage, Evry, FR;
Abstract
The present application provides polynucleotides comprising 5'-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5′-tail sequence segments. Cleavage of amplification products at the bond immediately 3′ to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.