The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 11, 2018

Filed:

May. 25, 2016
Applicant:

Board of Regents, the University of Texas System, Austin, TX (US);

Inventors:

Alan M. Lambowitz, Austin, TX (US);

Sabine Mohr, Austin, TX (US);

Georg Mohr, Austin, TX (US);

Eman Ghanem, Austin, TX (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 9/12 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1276 (2013.01); C12P 19/34 (2013.01); C12Y 207/07049 (2013.01); C07K 2319/00 (2013.01); C07K 2319/24 (2013.01);
Abstract

Stabilized reverse transcriptase fusion proteins including a thermostable reverse transcriptase connected to a stabilizer protein are described. Attaching the stabilizer protein to the thermostable reverse transcriptase stabilizes the fusion protein and can aid in its purification, provide increased solubility, allow for longer storage, or allow the fusion protein to be used under more rigorous conditions such as higher temperature. The stabilized reverse transcriptase fusion protein can also include a linker between the stabilizer protein and the thermostable reverse temperature. The stabilized reverse transcriptase fusion proteins are suitable for use in nucleic acid amplification methods such as the reverse transcription polymerase chain reaction and other applications involving cDNA synthesis.


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