The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 11, 2018
Filed:
Sep. 11, 2015
National Research Council of Canada, Ottawa, CA;
Jamshid Tanha, Orleans, CA;
National Research Council of Canada, Ottawa, CA;
Abstract
Polypeptides with desirable biophysical properties such as solubility, stability, high expression, monomericity, binding specificity or non-aggregation, including monomeric human Vs and Vs, are identified using a high throughput method for screening polypeptides, comprising the steps of obtaining a phage display library, allowing infection of a bacterial lawn by the library phage, and identifying phage which form larger than average plaques on the bacterial lawn. Sequences of monomeric human Vs and Vs are identified, which may be useful for immunotherapy or as diagnostic agents. Multimer complexes of human Vs and Vs are also identified. The Vs and Vs identified may be used to create further libraries for identifying additional polypeptides. Further, the Vs and Vs may be subjected to DNA shuffling to select for improved biophysical properties.