Method to determine the absorption coefficient in turbid media

Abstract

The invention relates to a method to determine the wavelength dependent absorption coefficient of a turbid medium using overlapping illumination-detection areas comprising the steps of a) retrieving a calibration spectrum (CA) from a reference measurement using a reference sample; b) carrying out a measurement on an actual sample for determining the absolute reflection spectrum (R) using a raw spectrum measured on the sample (S) and the calibration spectrum (C); C) using the absolute reflection spectrum (R) for determining the wavelength dependent absorption coefficient by minimizing the difference between the measured absolute reflection spectrum (R) and a model function (R). wherein the model function (R) is modelled using a predetermined equation based on prior knowledge of the combination of a dependence of the effective photon path length (L) on a scattering phase function (PF); a dependence of the absolute reflectance in the absence of absorption (R) on scattering phase function (PF). The invention further relates to a system and a computer program product for determining the wavelength dependent absorption coefficient of a turbid medium.