The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 20, 2018

Filed:

Apr. 02, 2012
Applicants:

Chieh Chang, San Jose, CA (US);

Rajiv Bharadwaj, Emeryville, CA (US);

Anup K. Singh, Danville, CA (US);

Aarthi Chandrasekaran, San Jose, CA (US);

Nathan J. Hillson, San Francisco, CA (US);

Inventors:

Chieh Chang, San Jose, CA (US);

Rajiv Bharadwaj, Emeryville, CA (US);

Anup K. Singh, Danville, CA (US);

Aarthi Chandrasekaran, San Jose, CA (US);

Nathan J. Hillson, San Francisco, CA (US);

Assignees:

The Regents of the University of California, Oakland, CA (US);

Sandia Corporation, Livermore, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/34 (2006.01); C12N 15/10 (2006.01); C12N 15/66 (2006.01); C12N 15/00 (2006.01); C12P 21/06 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1027 (2013.01); C12N 15/10 (2013.01); C12N 15/66 (2013.01);
Abstract

This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.


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