The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 13, 2018
Filed:
Mar. 13, 2014
Association for the Advancement of Tissue Engineering and Cell Based Technologies and Therapies, Braga, PT;
Raphael Faustino Canadas, Guimaraes, PT;
Joaquim Miguel Antunes De Oliveira, Braga, PT;
Alexandra Margarida Pinto Marques, Porto, PT;
Rui Luis Goncalves Dos Reis, Porto, PT;
Abstract
A rotational dual chamber bioreactor for cell culture in bi- and multi-layered scaffolds, in the context of tissue engineering (TE) and regenerative medicine strategies is disclosed, which comprises at least two dual culture chambers, a multiposition magnetic stirrer plate, and at least two flow pumps coupled to a monitor device to register biochemical parameters such as pH, pO, glucose and urea sensors and physical parameters like pressure. A central barrier, with a hole to insert the bilayer scaffold, is used to culture cells with different conditions in each compartment of the chamber, avoiding medium exchange. Each chamber can rotate 180° (horizontal), which is promoted by magnetic stirrers, the multiposition stirrer plate is adapted dimensionally to the culture plate dimensions and also allows 180° vertical rotation. Each dual chamber has detachable caps for the top and the bottom chambers, the top cap to compress the scaffold thereby providing compressive stimulus with torsion simultaneously.