The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 06, 2018

Filed:

May. 30, 2014
Applicant:

Allele Biotechnology & Pharmaceuticals, Inc., San Diego, CA (US);

Inventor:

Jiwu Wang, La Jolla, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 5/074 (2010.01); C12N 15/11 (2006.01); C07K 14/47 (2006.01); C07K 14/475 (2006.01);
U.S. Cl.
CPC ...
C12N 15/85 (2013.01); C07K 14/4702 (2013.01); C07K 14/475 (2013.01); C12N 5/0696 (2013.01); C12N 15/111 (2013.01); C07K 2319/01 (2013.01); C12N 2310/14 (2013.01); C12N 2320/30 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/605 (2013.01); C12N 2501/608 (2013.01); C12N 2506/1307 (2013.01); C12N 2510/00 (2013.01);
Abstract

The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.


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