The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 30, 2018

Filed:

Oct. 31, 2016
Applicant:

National University of Singapore, Singapore, SG;

Inventors:

Matthew Wook Chang, Singapore, SG;

Binbin Chen, Singapore, SG;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/81 (2006.01); C10L 1/04 (2006.01); C12P 5/02 (2006.01); C12N 9/88 (2006.01); C12N 9/00 (2006.01); C12N 9/08 (2006.01); C10G 3/00 (2006.01);
U.S. Cl.
CPC ...
C12Y 401/01072 (2013.01); C10G 3/00 (2013.01); C12N 9/0065 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12P 5/026 (2013.01); C12Y 111/01005 (2013.01); C12Y 111/01006 (2013.01); C12Y 403/01 (2013.01); C12Y 602/01003 (2013.01); C10G 2400/22 (2013.01); C10L 2290/26 (2013.01);
Abstract

Modifiedyeast that produce terminal alkenes are described. The modification of theyeast includes insertion of at least one heterologous fatty acid decarboxylase gene, deletion of FAA1 and FAA4, overexpression of HEM3, and triple-deletion of CTT1, CTA1 and CCP1. Methods of producing terminal alkenes by culturing and fermenting the modifiedyeast and optionally harvesting the terminal alkenes are also described. Mixtures of terminal alkenes produced by the modifiedyeast, and methods of metabolically engineering a yeast for optimizing overexpression of one or more alkenes are also described.


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