The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 23, 2018
Filed:
Nov. 13, 2014
Applicant:
The Brigham and Women's Hospital, Inc., Boston, MA (US);
Inventors:
Joshua Daniel Hutcheson, Allston, MA (US);
Elena Aikawa, Chestnut Hill, MA (US);
Masanori Aikawa, Chestnut Hill, MA (US);
Assignee:
The Brigham and Women's Hospital, Inc., Boston, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/02 (2006.01); C12Q 1/00 (2006.01); G01N 33/68 (2006.01); G01N 33/84 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6887 (2013.01); G01N 33/84 (2013.01); G01N 2333/78 (2013.01); G01N 2500/20 (2013.01); G01N 2800/323 (2013.01);
Abstract
Systems and methods for imaging of matrix vesicle-derived calcification are described. In one embodiment, the method comprises raising the pH of collagen stored in a solution, thereby causing the collagen to come out of the solution to form a network, adding matrix vesicles to the network, resulting in calcifications, and imaging the formation of the calcification. The calcification process can be imaged in real time by confocal or reflected light microscopy.