The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 16, 2018

Filed:

Aug. 06, 2015
Applicant:

Nugen Technologies, Inc., San Carlos, CA (US);

Inventors:

Jonathan Scolnick, San Francisco, CA (US);

Benjamin Schroeder, San Mateo, CA (US);

Douglas Amorese, Los Altos, CA (US);

Stephanie C. Huelga, Belmont, CA (US);

Assignee:

NuGEN Technologies, Inc., San Carlos, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G06F 19/22 (2011.01); C12Q 1/68 (2018.01); C12Q 1/6886 (2018.01); G06F 19/28 (2011.01); C12Q 1/6806 (2018.01); C12Q 1/689 (2018.01);
U.S. Cl.
CPC ...
G06F 19/22 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6886 (2013.01); G06F 19/28 (2013.01); C12Q 2600/158 (2013.01);
Abstract

Disclosed herein are methods, compositions and kits for quantitating one or more specific nucleic acids within a plurality of nucleic acids. In some embodiments, a sequencing library is constructed from enriched probe extension products specific for the specific nucleic acids and sequenced. In some embodiments, the resulting reads are used for removing duplicate reads. In some embodiments, counting of verified probes is used to quantitate or determine the number of specific nucleic acid molecules in the starting nucleic acid sample.


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