The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 16, 2018

Filed:

Mar. 20, 2014
Applicant:

Public University Corporation Yokohama City University, Yokohama-shi, Kanagawa, JP;

Inventors:

Hideki Taniguchi, Yokohama, JP;

Takanori Takebe, Yokohama, JP;

Shinji Kobayashi, Yokohama, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/077 (2010.01); C12N 5/078 (2010.01); A61L 27/38 (2006.01); C12N 5/00 (2006.01); A61K 35/32 (2015.01); A61L 27/36 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0062 (2013.01); A61K 35/32 (2013.01); A61L 27/3612 (2013.01); A61L 27/3808 (2013.01); A61L 27/3817 (2013.01); C12N 5/0655 (2013.01); G01N 33/5082 (2013.01); A61L 2430/06 (2013.01); A61L 2430/10 (2013.01); C12N 2501/105 (2013.01); C12N 2501/11 (2013.01); C12N 2501/113 (2013.01); C12N 2501/115 (2013.01); C12N 2501/119 (2013.01); C12N 2501/12 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/39 (2013.01); C12N 2502/28 (2013.01); C12N 2513/00 (2013.01);
Abstract

The present invention provides a method for preparing chondrocytes which enable construction of a cartilage tissue, the method being capable of solving the problems with conventional methods. A method for preparing chondrocytes, comprising co-culturing chondrogenic cells with vascular cells. A composition for cartilage regenerative therapy, comprising chondrocytes prepared by the above-described method. A method of screening for drugs effective as pharmaceuticals, comprising using chondrocytes prepared by the above-described method, a cartilage tissue formed from the chondrocytes and/or cells derived from the cartilage tissue. A method for preparing a matrix produced by chondrocytes, comprising using chondrocytes prepared by the above-described method, a cartilage tissue formed from the chondrocytes and/or cells derived from the cartilage tissue. A method for cartilage regeneration, comprising transplanting chondrocytes prepared by the above-described method into an organism to form a cartilage tissue.


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