The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 16, 2018

Filed:

Jun. 23, 2015
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

Mark A. Snyder, Oakland, CA (US);

Kimberly C. Brisack, San Francisco, CA (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 30/50 (2006.01); B01J 20/281 (2006.01); B01D 15/20 (2006.01); B01D 15/36 (2006.01); C01B 25/32 (2006.01); C01B 25/455 (2006.01); G01N 30/26 (2006.01); B01D 15/38 (2006.01); B01D 15/42 (2006.01); B01J 20/04 (2006.01); B01J 20/30 (2006.01); B01J 20/34 (2006.01);
U.S. Cl.
CPC ...
B01D 15/203 (2013.01); B01D 15/361 (2013.01); B01D 15/3847 (2013.01); C01B 25/32 (2013.01); C01B 25/327 (2013.01); C01B 25/455 (2013.01); G01N 30/26 (2013.01); G01N 30/48 (2013.01); G01N 30/50 (2013.01); B01J 20/048 (2013.01); B01J 20/3085 (2013.01); B01J 20/34 (2013.01);
Abstract

Disclosed are compositions and methods for regenerating or reducing the deterioration of an apatite solid surface during, or subsequent to, a chromatographic procedure for purifying a target molecule from a sample, by treating the apatite solid surface with a buffered calcium solution, followed by a phosphate buffered solution, followed by an alkaline hydroxide. The buffered calcium solution, phosphate buffered solution, and alkaline hydroxide can be applied subsequent to a bind and elute or flow through purification procedure. The methods provide an increase in resin mass and/or particle strength compared to prior methods.


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