The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 09, 2018

Filed:

Sep. 05, 2017
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

Stephen Hendricks, Los Gatos, CA (US);

David King, San Francisco, CA (US);

Lei Xi, Foster City, CA (US);

Marian Peris, San Mateo, CA (US);

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6853 (2018.01); C12P 19/34 (2006.01); C12Q 1/6874 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/46 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); C12P 19/34 (2013.01); C12Q 1/46 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6846 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01);
Abstract

In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5' phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.


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