The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 02, 2018
Filed:
Dec. 09, 2016
Applicant:
The Industry & Academic Cooperation IN Chungnam National University, Daejeon, KR;
Inventors:
Dong-Myung Kim, Daejeon, KR;
Taek Jin Kang, Seoul, KR;
Ho-Cheol Kim, Gyeonggi-do, KR;
Ki Baek Lee, Gyeonggi-do, KR;
Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/48 (2006.01); G01N 33/84 (2006.01); C07K 1/02 (2006.01); C12P 21/02 (2006.01); G01N 33/68 (2006.01);
U.S. Cl.
CPC ...
G01N 33/84 (2013.01); C07K 1/02 (2013.01); C12P 21/02 (2013.01); C12Y 401/01 (2013.01); G01N 33/6845 (2013.01);
Abstract
A method for cell-free protein synthesis is characterized in that pH is controlled by using an enzyme. For example, by using an amino acid decarboxylase, the pH is controlled according to removal of hydrogen ions that are produced during regeneration of ATP. The method for cell-free protein synthesis of the present invention has an advantage that not only the expression amount of protein is enhanced but also the expressed protein can be directly used for activity analysis without undergoing any separation or purification.