The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 02, 2018
Filed:
Jun. 16, 2016
Applicant:
University-industry Foundation, Yonsei University, Seoul, KR;
Inventors:
Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6834 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); C12N 15/1031 (2013.01); C12P 19/34 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6834 (2013.01);
Abstract
Provided is a method for stably synthesizing an error-free gene using a high-depth oligonucleotide tiling, which includes designing an oligonucleotide fragment by an over-overlapping method, synthesizing the oligonucleotide fragment using DNA microarray, retrieving error-free oligonucleotide fragments retrieved by next generation sequencing, and assembling the error-free oligonucleotide fragments.