The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 02, 2018
Filed:
Aug. 19, 2015
Arcticzymes As, Tromsø, NO;
Universitetet I Tromsø—norges Arktiske Universitet, Tromsø, NO;
Terese Solstad, Kvaløysletta, NO;
Elisabeth Lill Andreassen, Tromsø, NO;
Marit Sjo Lorentzen, Krokelvdalen, NO;
Olav Lanes, Tromsø, NO;
Morten Elde, Tromsø, NO;
Atle Noralf Larsen, Kvaløysletta, NO;
Yvonne Piotrowski, Tomasjord, NO;
Nils Peder Willassen, Tomasjord, NO;
ARCTICZYMES AS, Tromsø, NO;
UNIVERSITETET I TROMSØ—NORGES ARKTISKE UNIVERSITET, Tromsø, NO;
Abstract
The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID No. 1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCI, pH 8.5 at 25° C., 50 mM KCI and 5 mM MgCI; (ii) is substantially specific for single stranded DNA; and (iii) has a 3'-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.