The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 02, 2018

Filed:

Oct. 27, 2014
Applicant:

Agency for Science, Technology and Research, Singapore, SG;

Inventors:

Yun Shen Chan, Singapore, SG;

Huck Hui Ng, Singapore, SG;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0735 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0606 (2013.01); C12N 2501/155 (2013.01); C12N 2501/235 (2013.01); C12N 2501/724 (2013.01); C12N 2501/727 (2013.01); C12N 2501/999 (2013.01); C12N 2533/90 (2013.01);
Abstract

A method for culturing and maintaining a pluripotent stem cell in an undifferentiated state is provided. The method comprises culturing the pluripotent stem cell in a medium comprising an MEK inhibitor, a GSK3 inhibitor, a dual inhibitor of AMPK and/or BMP signaling and LIF. A cell produced by the method, cell culture medium and a kit for performing the method described is also provided.


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