The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 02, 2018

Filed:

Jan. 10, 2014
Applicant:

The Texas A&m University System, College Station, TX (US);

Inventors:

Zhilei Chen, College Station, TX (US);

Miguel A. Ramirez, Dunedin, NZ;

Dongli Guan, College Station, TX (US);

Assignee:

THE TEXAS A&M UNIVERSITY SYSTEM, College Station, TX (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/14 (2006.01); C12N 9/16 (2006.01); C07K 1/22 (2006.01); C07K 1/32 (2006.01);
U.S. Cl.
CPC ...
C07K 1/14 (2013.01); C07K 1/22 (2013.01); C07K 1/32 (2013.01); C12N 9/16 (2013.01); C07K 2319/20 (2013.01); C07K 2319/92 (2013.01); C12Y 301/00 (2013.01);
Abstract

The present invention includes methods, compositions, uses, and kits for purifying a protein of interest (POI) comprising contacting a first fusion protein comprising the POI fused to the C-terminus of an intein C-fragment with a second fusion protein comprising an intein N-fragment and a purification tag to form a complex between the first fusion protein and the second fusion protein, cleaving the POI from the intein C-fragment, wherein the protein is released from the complex; and isolating the POI; the present invention also includes fusion proteins and vector.


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