The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 25, 2018
Filed:
Oct. 29, 2008
Katja Friedrich, Nürnberg, DE;
Walter Gumbrecht, Herzogenaurach, DE;
Peter Paulicka, Erlangen, DE;
Manfred Stanzel, Erlangen, DE;
Renee Weber, Langenau-Albeck, DE;
Katja Friedrich, Nürnberg, DE;
Walter Gumbrecht, Herzogenaurach, DE;
Peter Paulicka, Erlangen, DE;
Manfred Stanzel, Erlangen, DE;
Renee Weber, Langenau-Albeck, DE;
Boehringer Ingelheim Vetmedica GmbH, Ingelheim am Rhein, DE;
Abstract
A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p]<Tm(K), and detecting a positive control signal; and optionally c) modifying the stringent conditions such that T[n]>Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)<T[mess]<Tm(Z). The method is suited in particular for the calibration and quality control of microarrays.