The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 25, 2018
Filed:
Mar. 14, 2014
Applicants:
Lyle J. Arnold, Poway, CA (US);
Norman C. Nelson, San Diego, CA (US);
Inventors:
Lyle J. Arnold, Poway, CA (US);
Norman C. Nelson, San Diego, CA (US);
Assignee:
AEGEA BIOTECHNOLOGIES, INC., Poway, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/6844 (2018.01);
U.S. Cl.
CPC ...
C12P 19/34 (2013.01); C12Q 1/6844 (2013.01);
Abstract
The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.