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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10078078 B1

PDFFull Text
Date of Patent:
Sep. 18, 2018

Filed:

Jul. 14, 2016

Bead incubation and washing on a droplet actuator

Applicant:

Advanced Liquid Logic, Inc., San Diego, CA (US);

Inventors:

Vamsee K. Pamula, Cary, NC (US);

Michael G. Pollack, Durham, NC (US);

Ramakrishna Sista, Cary, NC (US);

Arjun Sudarsan, Carlsbad, CA (US);

Assignee:

ADVANCED LIQUID LOGIC, INC., San Diego, CA (US);

Attorney:

Illumina, Inc.

Primary Examiner:

Brian J Sines

Int. Cl.
CPC ...
G01N 15/06 (2006.01); G01N 33/00 (2006.01); G01N 33/48 (2006.01); G01N 33/543 (2006.01); B01F 11/00 (2006.01); B01F 13/00 (2006.01); B01L 3/00 (2006.01); B03C 1/02 (2006.01); B03C 1/30 (2006.01); B03C 5/00 (2006.01); G01N 33/561 (2006.01); B03C 1/28 (2006.01); C12N 15/10 (2006.01); B01L 7/00 (2006.01); G01N 35/10 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54333 (2013.01); B01F 11/0071 (2013.01); B01F 13/0071 (2013.01); B01F 13/0076 (2013.01); B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); B01L 3/502784 (2013.01); B01L 3/502792 (2013.01); B03C 1/02 (2013.01); B03C 1/288 (2013.01); B03C 1/30 (2013.01); B03C 5/005 (2013.01); C12N 15/1013 (2013.01); G01N 33/561 (2013.01); B01L 7/52 (2013.01); B01L 7/525 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/089 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/0424 (2013.01); B01L 2400/0427 (2013.01); B01L 2400/0436 (2013.01); B01L 2400/0442 (2013.01); B03C 2201/18 (2013.01); B03C 2201/26 (2013.01); G01N 2035/1046 (2013.01); Y10T 436/11 (2015.01); Y10T 436/25 (2015.01); Y10T 436/2575 (2015.01); Y10T 436/25625 (2015.01);
Abstract

The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

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