The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 11, 2018

Filed:

Mar. 25, 2016
Applicant:

The Board of Trustees of the Leland Stanford Junior University, Palo Alto, CA (US);

Inventors:

Juan G. Santiago, Stanford, CA (US);

Alexandre Persat, Princeton, NJ (US);

Attorney:
Int. Cl.
CPC ...
G01N 27/447 (2006.01); C12Q 1/686 (2018.01); C12M 1/42 (2006.01); B01D 57/02 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
G01N 27/44765 (2013.01); B01D 57/02 (2013.01); C12M 1/42 (2013.01); C12P 19/34 (2013.01); C12Q 1/686 (2013.01); G01N 27/447 (2013.01); G01N 27/44721 (2013.01); G01N 27/44795 (2013.01); G01N 27/44791 (2013.01);
Abstract

Isotachophoresis (ITP) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an ITP zone, but the resulting product of the chemical reaction is separated from this ITP zone by the ITP process. In a second aspect, one or more reactants of a chemical reaction are confined to an ITP zone, and one or more other reactants of the chemical reaction are not confined to this ITP zone. In a third aspect, ITP is employed to confine at least one reactant of a chemical reaction to an ITP zone, and at least one reactant of the chemical reaction is delivered to the ITP zone in two or more discrete doses. These aspects are especially relevant to performing polymerase chain reactions using chemical denaturants as opposed to thermal cycling.


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