The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 04, 2018

Filed:

Jun. 03, 2014
Applicants:

Kaneka Corporation, Osaka-shi, Osaka, JP;

National Hospital Organization, Tokyo, JP;

Inventors:

Tomohisa Kato, Takasago, JP;

Yonehiro Kanemura, Osaka, JP;

Tomoko Shofuda, Osaka, JP;

Hayato Fukusumi, Osaka, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 5/074 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); G01N 33/5073 (2013.01); C12N 2500/25 (2013.01); C12N 2500/99 (2013.01); C12N 2501/115 (2013.01); C12N 2501/15 (2013.01);
Abstract

This invention relates to a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.


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