The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 04, 2018

Filed:

Dec. 12, 2013
Applicant:

National University Corporation Toyohashi University of Technology, Toyohashi-shi, JP;

Inventors:

Akira Mizuno, Nagoya, JP;

Rika Numano, Toyohashi, JP;

Hirofumi Kurita, Toyohasi, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12M 1/42 (2006.01); C12N 15/87 (2006.01); C12N 13/00 (2006.01);
U.S. Cl.
CPC ...
C12M 35/02 (2013.01); C12N 13/00 (2013.01); C12N 15/87 (2013.01);
Abstract

An exogenous substance transfer apparatus that introduces an exogenous substance into a small amount of cells by electrical action and a method for manufacturing exogenous substance-bearing cells at low cost are provided. In the introducing an exogenous substance like a gene into a cell, a cell suspension liquid that contains the cell and the exogenous substance is supplied to a container through an opening portion in an amount that is prepared such that a liquid droplet that is formed will not come into contact with a pair of electrodes simultaneously. The liquid droplet is formed in an oil stored in the container, without the cell suspension liquid mixing with the oil. When a direct current voltage is supplied to the electrodes from a power supply, the liquid droplet moves there-between, and the exogenous substance is introduced into the cell within the liquid droplet by electrical action.


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