The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 04, 2018

Filed:

Oct. 04, 2013
Applicant:

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Carlos Andres Aguilar, Boston, MA (US);

Tarun Kumar Jain, New York, NY (US);

Rohit N. Karnik, Cambridge, MA (US);

Peter A. Carr, Medford, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 61/44 (2006.01); B01D 69/02 (2006.01); G01N 33/487 (2006.01); B01D 63/00 (2006.01); B01D 67/00 (2006.01); B01D 71/02 (2006.01); G01N 15/12 (2006.01); G01N 15/10 (2006.01); B01D 69/12 (2006.01); B32B 37/26 (2006.01); G01N 27/447 (2006.01); B01L 3/00 (2006.01); G01N 15/00 (2006.01);
U.S. Cl.
CPC ...
B01D 69/02 (2013.01); B01D 61/44 (2013.01); B01D 63/005 (2013.01); B01D 67/009 (2013.01); B01D 67/0088 (2013.01); B01D 69/125 (2013.01); B01D 71/02 (2013.01); B32B 37/26 (2013.01); G01N 15/1056 (2013.01); G01N 15/12 (2013.01); G01N 27/44756 (2013.01); G01N 33/48721 (2013.01); B01D 2323/30 (2013.01); B01D 2325/028 (2013.01); B01D 2325/26 (2013.01); B01L 3/502761 (2013.01); G01N 2015/0038 (2013.01); G01N 2015/1081 (2013.01);
Abstract

Devices and methods integrate nanopore and microfluidic technologies for recording molecular characteristics of individual molecules such as, for example, biomolecules. Devices comprise a first substrate comprising a microchannel, a second substrate comprising a microchannel, the second substrate positioned below the first substrate, and a membrane having a thickness of about 0.3 nm to about 1 nm and comprising at least one nanopore, the membrane positioned between the first substrate and the second substrate, wherein a single nanopore of the membrane is constructed and arranged for electrical and fluid communication between the microchannel of the first substrate and the microchannel of the second substrate. To mitigate the effect of errors that occur during de novo DNA synthesis, longer DNA molecules are typically synthesized from shorter oligonucleotides by polymerase construction and amplification (PCA), or by other methods.


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