The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 04, 2018
Filed:
Oct. 19, 2015
Nanobiotix, Paris, FR;
Centre National DE LA Recherche Scientifique, Paris, FR;
Universite DE Bordeaux, Bordeaux, FR;
Agnés Pottier, Paris, FR;
Laurent Levy, Paris, FR;
Marie-Edith Meyre, Paris, FR;
Matthieu Germain, Champigny sur Marne, FR;
Cyril Lorenzato, Utrecht, NL;
Chrit Moonen, Stramproy, NL;
Pierre Smirnov, La Rochelle, FR;
NANOBIOTIX, Paris, FR;
UNIVERSITE DE BORDEAUX, Bordeaux, FR;
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris, FR;
Abstract
The invention pertains to a method of monitoring the membrane permeabilization of a liposome and the incidental release of a compound of interest. The method utilizes liposomes comprising a thermosensitive lipidic membrane encapsulating the product of interest and superparamagnetic nanoparticles having the electrostatic surface charge below −20 mV or above +20 mV when measured in an aqueous medium at physiological pH. In one embodiment, the method comprises the steps of: a) measuring relaxation time (T2*); b) heating the liposome at Tm or above Tm; c) measuring T2* after step b); d) obtaining the transverse relaxivity (r) values from the T2* values obtained from step a) and step c); and e) determining the ratio of rbefore and after the heating step b). A ratio above 1.5 indicates the liposome membrane permeabilization and the incidental release of the product of interest.