The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 28, 2018
Filed:
Nov. 03, 2017
Ultra-fast pathogen toxin detection assay based on microwave-accelerated metal-enhanced fluorescence
Applicant:
University of Maryland, Baltimore County, Baltimore, MD (US);
Inventor:
Chris D. Geddes, Bel-Air, MD (US);
Assignee:
UNIVERSITY OF MARYLAND, BALTIMORE COUNTY, Baltimore, MD (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01); C12Q 1/04 (2006.01);
U.S. Cl.
CPC ...
G01N 33/56911 (2013.01); C12Q 1/04 (2013.01); G01N 33/54313 (2013.01); G01N 33/54373 (2013.01); G01N 33/582 (2013.01); G01N 2333/32 (2013.01); G01N 2500/00 (2013.01);
Abstract
The present invention provides for a system and method to detect low levels of the anthrax protective antigen (PA) exotoxin in biological fluids, wherein the system uses a metal-enhanced fluorescence (MEF)-PA assay in combination with microwave-accelerated PA protein surface absorption. Microwave irradiation rapidly accelerates PA deposition onto the surface adjacent to deposited metallic particles and significantly speeding up the MEF-PA assay and resulting in a total assay run time of less than 40 min with an analytical sensitivity of less than 1 pg/ml PA.