The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 28, 2018

Filed:

Jun. 16, 2017
Applicant:

Life Technologies Corporation, Carlsbad, CA (US);

Inventors:

John Davidson, Guilford, CT (US);

Wolfgang Hinz, Killingworth, CT (US);

Jonathan Rothberg, Guilford, CT (US);

Richard Whitaker, Lynnfield, MA (US);

Assignee:

Life Technologies Corporation, Carlsbad, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12Q 1/6869 (2018.01); G01N 27/414 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12N 9/1252 (2013.01); C12Y 207/07007 (2013.01); G01N 27/4145 (2013.01);
Abstract

Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.


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