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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10053682 B1

PDFFull Text
Date of Patent:
Aug. 21, 2018

Filed:

Apr. 07, 2015

β-galactosidase mutant with high transglycosidase activity, and preparation method thereof and uses thereof

Applicant:

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, CN;

Inventors:

Wei Zhang, Beijing, CN;

Yuhong Zhang, Beijing, CN;

Bo Liu, Beijing, CN;

Ning Sun, Beijing, CN;

Jialin Zhang, Beijing, CN;

Assignee:

BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES, Beijing, CN;

Attorney:

Gokalp Bayramoglu

Primary Examiner:

Ganapathirama Raghu

Int. Cl.
CPC ...
C12N 9/38 (2006.01); C12N 9/24 (2006.01); C12N 9/14 (2006.01); C12P 21/06 (2006.01); C12P 19/34 (2006.01); C12N 1/00 (2006.01); C12N 15/00 (2006.01); C07H 21/04 (2006.01); C07K 1/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2471 (2013.01); C12Y 302/01023 (2013.01);
Abstract

The present invention relates to the field of genetic engineering and hereditary engineering. The present invention discloses a β-galactosidase (β-galactoside galactohydrolase, EC 3.2.1.23) mutant with high transglycosidase activity, which is obtained by single-site-saturation mutation of amino acid sequences of β-galactosidase fromand, with own signal peptides removed. The transglycosidase activity of the mutant is over 15% higher than that of wild types. Meanwhile, the present invention also discloses a DNA molecule which encodes the mutant, a recombinant expression vector containing the DNA molecule, and a host cell expressing the DNA molecule. In addition, the present invention also provides a method for preparing β-galactosidase mutant with high transglycosidase activity by using the recombinant expression vector and applications of the mutant, the DNA molecule, the recombinant expression vector and the host cell in preparation of β-galactosidase.

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