The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 14, 2018

Filed:

Aug. 21, 2015
Applicant:

Hoffmann-la Roche Inc., Little Falls, NJ (US);

Inventors:

Hubert Kettenberger, Munich, DE;

Stefan Klostermann, Neuried, DE;

Ulrich Kohnert, Habach, DE;

Sebastian Neumann, Weilheim, DE;

Assignee:

HOFFMANN-LA ROCHE INC., Little Falls, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 16/00 (2006.01); C07K 16/28 (2006.01); C07K 16/46 (2006.01);
U.S. Cl.
CPC ...
C07K 16/2866 (2013.01); C07K 16/00 (2013.01); C07K 16/2875 (2013.01); C07K 16/461 (2013.01); C07K 2317/24 (2013.01); C07K 2317/567 (2013.01);
Abstract

Herein is reported a method for reducing the aggregation of an immunoglobulin in solution comprising the steps of i) comparing the amino acid sequence of the fourth framework region of the heavy chain of an antibody with a reference or germline sequence and determining whether one or more threonine residues and/or serine residues have been replaced by a different amino acid residue, and ii) modifying the amino acid sequence of the immunoglobulin by reverting the exchanged threonine residues and/or serine residues back to threonine or serine of the reference or germline sequence and thereby reducing the aggregation of an immunoglobulin in solution.


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