The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 07, 2018

Filed:

Jul. 13, 2016
Applicant:

Qyuns Therapeutics Co., Ltd., Taizhou, CN;

Inventor:

Guo-liang Yu, Hillsborough, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G06F 19/22 (2011.01); C07K 16/18 (2006.01); C07K 16/06 (2006.01); C40B 30/04 (2006.01); C07K 16/28 (2006.01); C07K 14/725 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
G06F 19/22 (2013.01); C07K 14/7051 (2013.01); C07K 16/2827 (2013.01); G01N 33/5005 (2013.01); C07K 2317/14 (2013.01); C07K 2317/24 (2013.01); C07K 2317/565 (2013.01); C07K 2317/76 (2013.01); C40B 30/04 (2013.01);
Abstract

Provided are methods for identifying antibodies that bind to an antigen in primates. In certain embodiments, the methods comprise: sequencing the cDNA prepared from a combined population of antibody-producing B-cells to obtain a plurality of antibody heavy chain sequences and a plurality of antibody light chain sequences; grouping the obtained antibody heavy chain sequences into heavy chain groups based on their CDR3 sequences; grouping the obtained antibody light chain sequences into light chain groups based on their CDR3 sequences; pairing the heavy chain groups with the light chain groups based on the number of antibody heavy chain or light chain sequences in each group; pairing one antibody heavy chain sequence and one light chain sequence in each paired heavy chain group and light chain group; and testing candidate antibodies comprising the paired heavy chain and light chain sequences for binding to the antigen.


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