The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 07, 2018

Filed:

Jan. 19, 2016
Applicant:

University of Wyoming, Laramie, WY (US);

Inventors:

Mark Gomelsky, Laramie, WY (US);

Min-Hyung Ryu, Laramie, WY (US);

Assignee:

University of Wyoming, Laramie, WY (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/00 (2006.01); C12N 15/62 (2006.01); A61K 48/00 (2006.01); A61N 5/00 (2006.01); G01N 33/52 (2006.01); C12N 9/88 (2006.01); C07K 14/195 (2006.01); A61K 38/16 (2006.01); A61K 38/51 (2006.01); A61K 41/00 (2006.01); A61N 5/06 (2006.01); A61K 31/711 (2006.01); A61K 31/713 (2006.01); C07K 7/06 (2006.01); C12N 9/50 (2006.01); C12N 9/64 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 9/88 (2013.01); A61K 31/711 (2013.01); A61K 31/713 (2013.01); A61K 38/164 (2013.01); A61K 38/51 (2013.01); A61K 41/00 (2013.01); A61N 5/062 (2013.01); C07K 7/06 (2013.01); C07K 14/195 (2013.01); C12N 9/50 (2013.01); C12N 9/6472 (2013.01); C12N 9/6475 (2013.01); C12N 15/62 (2013.01); C12Y 114/14 (2013.01); C12Y 304/22056 (2013.01); C12Y 406/01001 (2013.01); C12Y 406/01002 (2013.01); G01N 33/5023 (2013.01); G01N 33/5088 (2013.01); A61K 48/00 (2013.01); A61N 2005/0659 (2013.01); C07K 2319/00 (2013.01); C07K 2319/60 (2013.01);
Abstract

Methods and constructs are provided for controlling processes in live animals, plants or microbes via genetically engineered near-infrared light-activated or light-inactivated proteins including chimeras including the photosensory modules of bacteriohytochromes and output modules that possess enzymatic activity and/or ability to bind to DNA, RNA, protein, or small molecules. DNA encoding these proteins are introduced as genes into live animals, plants or microbes, where their activities can be turned on by near-infrared light, controlled by the intensity of light, and turned off by near-infrared light of a different wavelength than the activating light. These proteins can regulate diverse cellular processes with high spatial and temporal precision, in a nontoxic manner, often using external light sources. For example, near-infrared light-activated proteins possessing nucleotidyl cyclase, protein kinase, protease, DNA-binding and RNA-binding activities are useful to control signal transduction, cell apoptosis, proliferation, adhesion, differentiation and other cell processes.


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