The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 07, 2018

Filed:

Mar. 11, 2014
Applicants:

The Brigham and Women's Hospital, Inc., Boston, MA (US);

Massachusetts Institute of Technology, Cambridge, MA (US);

Inventors:

Jeffrey Michael Karp, Brookline, MA (US);

Xiaolei Yin, Quincy, MA (US);

Marc David Succi, Boston, MA (US);

Robert Samuel Langer, Newton, MA (US);

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
C12N 5/068 (2013.01); C12N 5/0679 (2013.01); C12N 2501/065 (2013.01); C12N 2501/11 (2013.01); C12N 2501/155 (2013.01); C12N 2501/415 (2013.01); C12N 2501/42 (2013.01); C12N 2501/727 (2013.01); C12N 2501/73 (2013.01); C12N 2513/00 (2013.01);
Abstract

Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation. A single layer of epithelial cells that actively self-renews and is organized into crypts and villi clothes the intestine. It has been recently shown that the renewal of intestinal epithelium is driven by Lgr5+ intestinal stem cells (ISC) that reside at the base of these crypts (Barker et al., 2007). Lgr5+ stem cells can be isolated and cultured in vitro to form organoids containing crypt-vcllus structures that recapitulates the native intestinal epithelium (Sato et al., 2009).


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