The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 07, 2018

Filed:

Jun. 21, 2010
Applicants:

Camilo Colaco, Cambridge, GB;

Colin Richard Bignell, Cambridge, GB;

Inventors:

Camilo Colaco, Cambridge, GB;

Colin Richard Bignell, Cambridge, GB;

Assignee:

Immunobiology Limited, Cambridge, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/18 (2006.01); A61K 39/00 (2006.01); A61K 39/04 (2006.01); A61K 39/09 (2006.01); A61K 39/095 (2006.01); A61K 39/12 (2006.01); A61K 39/145 (2006.01); A61K 39/29 (2006.01); A61K 39/385 (2006.01); A61K 39/39 (2006.01); C07K 14/195 (2006.01);
U.S. Cl.
CPC ...
C07K 1/18 (2013.01); A61K 39/0011 (2013.01); A61K 39/04 (2013.01); A61K 39/092 (2013.01); A61K 39/095 (2013.01); A61K 39/12 (2013.01); A61K 39/145 (2013.01); A61K 39/29 (2013.01); A61K 39/385 (2013.01); A61K 39/39 (2013.01); C07K 14/195 (2013.01); A61K 2039/55516 (2013.01); A61K 2039/605 (2013.01); C12N 2710/14043 (2013.01); C12N 2760/16134 (2013.01); C12N 2770/24234 (2013.01); Y02A 50/394 (2018.01); Y02A 50/397 (2018.01); Y02A 50/403 (2018.01); Y02A 50/464 (2018.01); Y02A 50/466 (2018.01); Y02A 50/478 (2018.01);
Abstract

The present invention provides an improved method for the purification of a mixture of complexes comprising a stress protein complexed to a peptide or peptide fragment from a source mixture, typically a cell lysate. The method of the invention provides for protein complexes to be purified using ion exchange chromatography based methods, wherein a modified buffer solution is used which results in the purified stress protein complexes being more immunogenic than protein complexes obtained using conventional methodology. The purified complexes can be used to produce improved vaccine preparations which elicit enhanced immune responses in the subjects to whom the vaccine compositions are administered.


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