The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 31, 2018

Filed:

Oct. 03, 2013
Applicant:

Gyros Patent Ab, Uppsala, SE;

Inventors:

Mats Inganäs, Uppsala, SE;

Pirjo Lehtonen, Uppsala, SE;

Assignee:

GYROS Patent AB, Uppsala, SE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/537 (2006.01); G01N 33/543 (2006.01); G01N 33/53 (2006.01);
U.S. Cl.
CPC ...
G01N 33/54393 (2013.01); G01N 33/5306 (2013.01); G01N 33/5375 (2013.01); G01N 2333/195 (2013.01);
Abstract

A method of quantitatively determining an analyte in a fluid sample by an immunoassay comprises binding of the analyte to a ligand capable of specifically binding to the analyte, wherein at least part of the analyte is present as an analyte complex is disclosed. The method comprises the steps of: a) subjecting the sample to a first acidic pH to at least substantially dissociate any analyte complex present and provide substantially all analyte in free form, b) raising the first acidic pH to a second acidic pH where re-formation of complexes is prevented but where binding of analyte to the ligand is permitted, and c) determining the binding of analyte to the ligand to quantitatively determine the analyte in the sample.


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