The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 31, 2018

Filed:

Jan. 15, 2015
Applicants:

Abbott Japan Co., Ltd., Chiba, JP;

Tokyo Institute of Technology, Tokyo, JP;

Inventors:

Ken Komiya, Tokyo, JP;

Makoto Komori, Matsudo, JP;

Toru Yoshimura, Matsudo, JP;

Assignees:

Abbott Laboratories, Abbott Park, IL (US);

Tokyo Institute of Technology, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12Q 1/70 (2006.01); C12Q 1/689 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/682 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/707 (2013.01); C12Q 1/682 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/703 (2013.01); C12Q 1/706 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/178 (2013.01);
Abstract

Disclosed are methods for detecting a target nucleic acid in sample, which include contacting the sample with an oligonucleotide having a hairpin structure, where the oligonucleotide includes, in the 5' to 3′ direction, an arbitrary sequence, an endonuclease recognition site for a nicking reaction, a sequence complementary to the arbitrary sequence, and a sequence complementary to the target nucleic acid; a polymerase; and an endonuclease capable of nicking the endonuclease recognition site. The disclosure also provides compositions and kits comprising an oligonucleotide having a hairpin structure, where the oligonucleotide includes, in the 5′ to 3′ direction an arbitrary sequence, an endonuclease recognition site for a nicking reaction, a sequence complementary to the arbitrary sequence, and a sequence complementary to a target nucleic acid.


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