The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 24, 2018

Filed:

May. 10, 2016
Applicant:

General Electric Company, Schenectady, NY (US);

Inventors:

Patrick McCoy Spooner, Albany, NY (US);

Peter James Tatnell, Caerphilly, GB;

Jeffrey Kenneth Horton, Vale of Glamorgan, GB;

John Richard Nelson, Clifton Park, NY (US);

Michael John Gerdes, Albany, NY (US);

Suzana Kiel, Göttingen, DE;

Ralf Lenigk, Niskayuna, NY (US);

Alexander Schenk, Dassel, DE;

Wei Sun, Göttingen, DE;

Assignee:

GENERAL ELECTRIC COMPANY, Schenectady, NY (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); G01N 33/53 (2006.01); C12Q 1/6804 (2018.01); G01N 27/447 (2006.01); G01N 33/68 (2006.01); C12Q 1/6888 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1013 (2013.01); C12N 15/101 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6888 (2013.01); G01N 27/44743 (2013.01); G01N 27/44747 (2013.01); G01N 33/5308 (2013.01); G01N 33/689 (2013.01); C12Q 2600/124 (2013.01);
Abstract

The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.


Find Patent Forward Citations

Loading…