The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 24, 2018

Filed:

Mar. 12, 2013
Applicant:

Hitachi, Ltd., Chiyoda-ku, Tokyo, JP;

Inventors:

Masataka Shirai, Tokyo, JP;

Hideki Kambara, Tokyo, JP;

Kiyomi Taniguchi, Tokyo, JP;

Maiko Tanabe, Tokyo, JP;

Assignee:

Hitachi, Ltd., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6874 (2018.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1003 (2013.01); B01L 3/502761 (2013.01); B01L 7/52 (2013.01); C12N 15/1096 (2013.01); C12Q 1/6874 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0851 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/1805 (2013.01); B01L 2400/0421 (2013.01);
Abstract

In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.


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