The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 24, 2018

Filed:

Jan. 21, 2015
Applicant:

Novozymes A/s, Bagsvaerd, DK;

Inventors:

Tomoko Matsui, Chiba, JP;

Akihiko Yamagishi, Tokyo, JP;

Assignee:

NOVOZYMES A/S, Bagsvaerd, DK;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/44 (2006.01); C12N 9/42 (2006.01); C12N 9/24 (2006.01); C12P 19/16 (2006.01); C12P 21/06 (2006.01); C12P 19/34 (2006.01); C12N 15/00 (2006.01); C12N 1/20 (2006.01); C12N 1/00 (2006.01); C07H 21/04 (2006.01); C07K 1/00 (2006.01); C12P 19/02 (2006.01); C12P 7/06 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2457 (2013.01); C12P 7/06 (2013.01); C12P 19/02 (2013.01); C12P 19/16 (2013.01); C12Y 302/01041 (2013.01); C12P 2203/00 (2013.01); Y02E 50/17 (2013.01);
Abstract

The present invention relates to pullulanase variants having increased thermoactivity and comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The variants are mainly derived from pullulanases fromor hybrid pullulanases.


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