The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 17, 2018
Filed:
Apr. 28, 2011
Applicant:
Jonathan Peter Clark, Kelty, GB;
Inventor:
Jonathan Peter Clark, Kelty, GB;
Assignee:
ITI Scotland Limited, Glasgow, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); C12Q 1/25 (2006.01);
U.S. Cl.
CPC ...
G01N 33/68 (2013.01); C12Q 1/25 (2013.01); G01N 2333/9015 (2013.01);
Abstract
The present invention related to a method of assaying ubiquitination in a sample by combining ubiquitin and two or more of E1, E2, E3 and a substrate protein in a sample under conditions suitable for ubiquitination to take place, exposing the sample with a labelled binding partner which is specific for the ubiquitin and measuring the amount of labelled ubiquitin bound to any one of the components in the sample, wherein one or more of the components in the sample comprises an immobilization tag which facilitates its immobilization onto a solid surface.