The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 17, 2018
Filed:
Oct. 14, 2014
Bgi Shenzhen Co., Limited, Shenzhen, CN;
Chunyu Geng, Shenzhen, CN;
Rongrong Guo, Shenzhen, CN;
Ruoying Chen, Shenzhen, CN;
Lingyu He, Shenzhen, CN;
Wenwei Zhang, Shenzhen, CN;
Hui Jiang, Shenzhen, CN;
MGI Tech Co., Ltd., Shenzhen, CN;
Abstract
Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagent kit thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first PCR reaction, wherein the 5' end of one of the primers has a first affinity tag, resulting in a product with two ends connected to different linker sequences; binding the product to a solid vector having a second affinity tag; degenerating and separating single strands having no affinity tag; and cyclizing the single strands.