The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 17, 2018
Filed:
Sep. 24, 2014
Applicant:
Thermo Fisher Scientific Baltics Uab, Vilnius, LT;
Inventors:
Assignee:
Thermo Fisher Scientific Baltics UAB, Vilnius, LT;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12N 11/18 (2006.01); C12N 9/96 (2006.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
C12N 9/96 (2013.01); C12N 9/1205 (2013.01); C12N 9/1241 (2013.01); C12N 9/1252 (2013.01); C12N 9/22 (2013.01); C12N 11/18 (2013.01); C12Y 207/01078 (2013.01);
Abstract
Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic DNA polymerase lacking 5'-3′ and 3′-5′ exonuclease activity premixed with T4 DNA polymerase, Klenow fragment and T4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform DNA blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps. Among other benefits, the mixture of different enzymes, buffers and nucleoside triphosphates is stable during prolonged storage.