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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10018630 B1

PDFFull Text
Date of Patent:
Jul. 10, 2018

Filed:

Sep. 07, 2012

Cancer stem cell isolation

Applicants:

Tatsumi Yamazaki, Tokyo, JP;

Hisafumi Okabe, Shizuoka, JP;

Shinta Kobayashi, Helios, SG;

Takeshi Watanabe, Shizuoka, JP;

Koichi Matsubara, Helios, SG;

Atsuhiko Kato, Shizuoka, JP;

Masami Suzuki, Shizuoka, JP;

Inventors:

Tatsumi Yamazaki, Tokyo, JP;

Hisafumi Okabe, Shizuoka, JP;

Shinta Kobayashi, Helios, SG;

Takeshi Watanabe, Shizuoka, JP;

Koichi Matsubara, Helios, SG;

Atsuhiko Kato, Shizuoka, JP;

Masami Suzuki, Shizuoka, JP;

Assignees:

CHUGAI SEIYAKU KABUSHIKI KAISHA, Tokyo, JP;

PHARMALOGICALS RESEARCH PTE. LTD., Helios, SG;

Attorney:

Klarquist Sparkman, LLP

Primary Examiner:

Yan Xiao

Int. Cl.
CPC ...
G01N 33/574 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01); C12N 5/095 (2010.01); A61K 31/4745 (2006.01); A61K 31/513 (2006.01); A61K 31/555 (2006.01); A61K 31/7088 (2006.01); A61K 39/00 (2006.01); A61K 39/395 (2006.01); A61K 45/06 (2006.01); C07K 16/18 (2006.01); A61K 31/7105 (2006.01); A61K 31/713 (2006.01); C07K 16/30 (2006.01);
U.S. Cl.
CPC ...
G01N 33/574 (2013.01); A61K 31/4745 (2013.01); A61K 31/513 (2013.01); A61K 31/555 (2013.01); A61K 31/7088 (2013.01); A61K 31/713 (2013.01); A61K 31/7105 (2013.01); A61K 39/0011 (2013.01); A61K 39/3955 (2013.01); A61K 45/06 (2013.01); C07K 16/18 (2013.01); C07K 16/30 (2013.01); C12N 5/0695 (2013.01); G01N 33/5073 (2013.01); G01N 33/56966 (2013.01); G01N 2500/04 (2013.01);
Abstract

An objective of the present invention is to provide: a cancer stem cell isolated using a cell marker; a substantively homogeneous cancer stem cell population including said cancer stem cell; and a method of preparing said cancer stem cell population. Another objective of the present invention is to provide: a method for separating cancer stem cells with a high proliferative potential and those with a low proliferative potential; a method for inducing cancer stem cells to have a different proliferative potential; and cancer stem cells separated or induced by these separation or induction methods. A further objective of the present invention is to provide: a method of screening for pharmaceuticals using said cancer stem cell or cancer stem cell population; a method for detecting the presence of said cancer stem cell or cancer stem cell population and for identifying or quantifying the same. The present inventors discovered that highly pure colon cancer stem cells (CSC) can be obtained in a large quantity, and identified two types of states of colon CSCs distinguishable through Lgr5 expression.

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