The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 10, 2018
Filed:
Jul. 30, 2013
Bgi Genomics Co., Limited, Shenzhen, Guangdong, CN;
Xiaoyu Pan, Shenzhen, CN;
Jing Guo, Shenzhen, CN;
Hui Jiang, Shenzhen, CN;
Fang Chen, Shenzhen, CN;
Shengpei Chen, Shenzhen, CN;
Shida Zhu, Shenzhen, CN;
Fan Fan, Shenzhen, CN;
Jialou Zhu, Shenzhen, CN;
BGI GENOMICS CO., LIMITED, Shenzhen, Guangdong, CN;
Abstract
The present invention provides a method for determining the nucleic acid composition in a total nucleic acid mixture comprising a first nucleic acid and a second nucleic acid. The method comprises: 1) treating the total nucleic acid mixture with a bisulfate, to convert the non-methylated cytosine in the total nucleic acid mixture into uracil, and obtain a converted total nucleic acid mixture; 2) subjecting the converted total nucleic acid mixture to multiplexed fluorescent quantitative PCR using a first set of amplification primers and a second set of amplification primers; and 3) based on the ratio R of the methylated amplification product to the non-methylated amplification product of the predetermined nucleic acid fragment, a methylation proportion Mof the predetermined nucleic acid fragment in the first nucleic acid, and a methylation proportion Mof the predetermined nucleic acid fragment in the second nucleic acid, determining the nucleic acid composition in the total nucleic acid mixture.