The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 10, 2018
Filed:
Dec. 13, 2016
Applicant:
Institut Pasteur, Paris, FR;
Inventors:
Philippe Despres, La Garenne-Colombes, FR;
Sylvie Paulous, Sarcelles, FR;
Elodie Crublet, L'Albenc, FR;
Assignee:
INSTITUT PASTEUR, Paris, FR;
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01); C12N 9/96 (2006.01); C12P 19/34 (2006.01); C12N 15/00 (2006.01); C12N 5/10 (2006.01); C12N 5/07 (2010.01); G01N 33/53 (2006.01); C12Q 1/70 (2006.01); A61K 39/385 (2006.01); A61K 39/12 (2006.01); C07K 1/00 (2006.01); C12P 21/08 (2006.01); C07K 16/00 (2006.01); C12N 15/62 (2006.01); C12N 15/85 (2006.01); C12N 5/09 (2010.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C12N 15/625 (2013.01); C12N 5/0601 (2013.01); C12N 5/0693 (2013.01); C12N 9/1007 (2013.01); C12N 9/96 (2013.01); C12N 15/85 (2013.01); C12P 21/02 (2013.01); C12Y 201/01063 (2013.01); C07K 2319/00 (2013.01); C07K 2319/055 (2013.01); C07K 2319/20 (2013.01); C07K 2319/21 (2013.01); C07K 2319/50 (2013.01); C12N 2015/8518 (2013.01); C12N 2510/02 (2013.01); Y02A 50/51 (2018.01); Y02A 50/53 (2018.01); Y02A 50/60 (2018.01);
Abstract
The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein.